Jang, Sung Key, Ph.D.

Professor
Department of Life Science
Division of Molecular and Life Sciences
Molecular Virology

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Publications Abstract
E-mail sungkey@postech.ac.kr
Phone +82-54-279-2298(office)
          +82-54-279-5985(lab.)
Laboratory Molecular Virology lab.

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Profile |  Research Interests  |  Selected Publications

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1989
1989-1991

Ph.D., State University of New York, Stony Brook
Post-doctoral Associate, State University of New York, Stony Brook

 

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Molecular Virology I
Cap-independent translation of eukaryotic mRNAs requires a specific RNA structure, named `internal ribosomal entry site (IRES)', in the mRNA and IRES-specific cellular factors along with basic translational machinery. We are investigating molecular mechanism of translation via IRESes by analyzing cis-acting elements and cellular factors interacting with IRESes of viral ［encephalomyocarditis virus, poliovirus and hepatitis C virus (HCV)］ and cellular (Bip, c-myc) IRESes. The roles of IRES-dependent translation in the cell cycle and differentiation process are also under investigation.
HCV is an enveloped RNA virus containing a single positive sense RNA in a virion particle. The genomic RNA encodes a polyprotein (~3010 amino acids) with the following gene order: 5'-C-E1-E2-NS2-NS3-NS4A-NS4B-NS5A-NS5B-3'. During and/or after synthesis the polyprotein is processed into functional proteins by host- and virus-encoded proteases. Core (C) and envelope proteins (E1 and E2) are believed to compose the structural elements of the virion particle. NS2, NS3, and NS4A are involved in the proteolytic processing of the HCV polyprotein. RNA-dependent RNA polymerase and RNA helicase activities are assigned to NS5B and the C-terminal two-thirds of NS3, respectively. We are investigating the biochemical properties of NS3, which contains both protease and helicase domains. We are trying to develop anti-HCV drug based on HCV protease inhibitor activity. Currently, modulation of cellular function by HCV proteins is also under investigation.

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  1. Park S, Yang JS, Shin YE, Park J, Jang SK, Kim S. Protein localization as a principal feature of the etiology and comorbidity of genetic diseases. Mol Syst Biol. 2011 May 24;7:494.
  2. Kim JH, Park SM, Park JH, Keum SJ, Jang SK. eIF2A mediates translation of hepatitis C viral mRNA under stress conditions. EMBO J. 2011 May 10. [Epub ahead of print]
  3. Lim C, Lee J, Choi C, Kilman VL, Kim J, Park SM, Jang SK, Allada R, Choe J. The novel gene twenty-four defines a critical translational step in the Drosophila clock. Nature. 2011 Feb 17;470(7334):399-403.
  4. Kim HW, Ha SH, Lee MN, Huston E, Kim DH, Jang SK, Suh PG, Houslay MD, Ryu SH. Cyclic AMP controls mTOR through regulation of the dynamic interaction between Rheb and phosphodiesterase 4D. Mol Cell Biol. 2010 Nov;30(22):5406-20.
  5. Kim KM, Cho H, Choi K, Kim J, Kim BW, Ko YG, Jang SK, Kim YK. A new MIF4G domain-containing protein, CTIF, directs nuclear cap-binding protein CBP80/20-dependent translation. Genes Dev. 2009 Sep 1;23(17):2033-45.
  6. Park S, Yang JS, Jang SK, Kim S. Construction of functional interaction networks through consensus localization predictions of the human proteome. J Proteome Res. 2009 Jul;8(7):3367-76.
  7. Chang JH, Cho YH, Sohn SY, Choi JM, Kim A, Kim YC, Jang SK, Cho Y. Crystal structure of the eIF4A-PDCD4 complex. Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3148-53.
  8. Hwang B, Lim JH, Hahm B, Jang SK, Lee SW. hnRNP L is required for the translation mediated by HCV IRES. Biochem Biophys Res Commun. 2009 Jan 16;378(3):584-8.
  9. Paek KY, Kim CS, Park SM, Kim JH, Jang SK. RNA-binding protein hnRNP D modulates internal ribosome entry site-dependent translation of hepatitis C virus RNA. J Virol. 2008 Dec;82(24):12082-93.
  10. Jeong HS, Seo YJ, Bang EK, Hwang GT, Jung JH, Jang SK, Kim BH. Cholesterol-linked pyrene excimer molecular beacon with enhanced cell permeability. Nucleic Acids Symp Ser (Oxf). 2008;(52):351-2.
  11. Cho H, Lee HC, Jang SK, Kim YK. Iron increases translation initiation directed by internal ribosome entry site of hepatitis C virus. Virus Genes. 2008 Oct;37(2):154-60.
  12. Chung KM, Cha SS, Jang SK. A novel function of karyopherin beta3 associated with apolipoprotein A-I secretion. Mol Cells. 2008 Sep 30;26(3):291-8
  13. Rho J, Ryu JS, Hur W, Kim CW, Jang JW, Bae SH, Choi JY, Jang SK, Yoon SK. Hepatitis C virus (HCV) genotyping by annealing reverse transcription-PCR products with genotype-specific capture probes. J Microbiol. 2008 Feb;46(1):81-7.
  14. Lee SH, Song R, Lee MN, Kim CS, Lee H, Kong YY, Kim H, Jang SK. A molecular chaperone glucose-regulated protein 94 blocks apoptosis induced by virus infection. Hepatology. 2008 Mar;47(3):854-66.
  15. Kwon MC, Koo BK, Moon JS, Kim YY, Park KC, Kim NS, Kwon MY, Kong MP, Yoon KJ, Im SK, Ghim J, Han YM, Jang SK, Shong M, Kong YY. Crif1 is a novel transcriptional coactivator of STAT3. EMBO J. 2008 Feb 20;27(4):642-53.
  16. Kim WJ, Kim JH, Jang SK. Anti-inflammatory lipid mediator 15d-PGJ2 inhibits translation through inactivation of eIF4A. EMBO J. 2007 Dec 12;26(24):5020-32.
  17. Kim JE, Ryu I, Kim WJ, Song OK, Ryu J, Kwon MY, Kim JH, Jang SK. Proline-rich transcript in brain protein induces stress granule formation. Mol Cell Biol. 2008 Jan;28(2):803-13.
  18. Kim CS, Jung JH, Wakita T, Yoon SK, Jang SK. Monitoring the antiviral effect of alpha interferon on individual cells. J Virol. 2007 Aug;81(16):8814-20.
  19. You KT, Li LS, Kim NG, Kang HJ, Koh KH, Chwae YJ, Kim KM, Kim YK, Park SM, Jang SK, Kim H. Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors. PLoS Biol. 2007 May;5(5):e109.
  20. Kim TD, Woo KC, Cho S, Ha DC, Jang SK, Kim KT. Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production. Genes Dev. 2007 Apr 1;21(7):797-810.
  21. Kim CS, Seol SK, Song OK, Park JH, Jang SK. An RNA-binding protein, hnRNP A1, and a scaffold protein, septin 6, facilitate hepatitis C virus replication. J Virol. 2007 Apr;81(8):3852-65.
  22. Cho S, Park SM, Kim TD, Kim JH, Kim KT, Jang SK. BiP internal ribosomal entry site activity is controlled by heat-induced interaction of NSAP1. Mol Cell Biol. 2007 Jan;27(1):368-83.
  23. Kim KM, Kwon SN, Kang JI, Lee SH, Jang SK, Ahn BY, Kim YK. Hepatitis C virus NS2 protein activates cellular cyclic AMP-dependent pathways. Biochem Biophys Res Commun. 2007 May 18;356(4):948-54.

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Division of Molecular & Life Sciences| POSTECH