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Research Interests
Materials and Bioinorganic Chemistry
Research
interests in this laboratory are focused on the
development of the DNA chip technology and DNA-hydrolyzing
metal catalysts.
DNA chips are glass surfaces
bearing arrays of DNA fragments at discrete addresses,
of which the fragments are available for the hybridization.
The DNA spots on the chip are hybridized to a complex
sample of fluorescently labeled DNA or RNA in solution.
DNA chips are commonly used either to monitor expression
of m-RNAs or to detect DNA sequence polymorphisms
or mutations in genomic DNA. The technology holds
great promise for identifying gene polymorphisms
that predispose man to disease, gene regulation
events involved in disease progression, and more-effective
disease treatments.
Phosphate esters and anhydrides
dominate the living world. The genetic materials,
DNA and RNA are phosphate diesters. As biochemistry
and genomic engineering field grow very rapidly
in recent years, many scientists are interested
in developing the artificial restriction enzymes
which will overcome limitations of the natural ones.
In order to create artificial restriction enzymes,
metal centers should be capable of hydrolyzing phosphodiester
of DNA at a resonable rate (in a few minutes). In
this laboratory we are developing lanthanide complexes
which can hydrolyze phosphate backbone of DNA like
active centers of the artificial restriction enzymes.
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Selected
Publications
J. H. Moon, J. H. Kim, K.-J. Kim, T.-H. Kang,
B. Kim, C.-H. Kim, J. H. Hahn, J. W. Park (1997)
Absolute surface density of the amine group of the
aminosilylated thin layers: UV-vis
spectroscopy,
second harmonic generation, and synchrotron-radiation
photoelectron spectroscopy study. Langmuir,
13, 16, 4305-4310, American Chemical Society
Joong Ho Moon, Ki-Jeong Kim, Tai-Hee
Kang, Bohgsoo Kim, Heon Kang,and Joon Won Park(1998)Selective
Cleavage of the Nitro Group from a Nitrophenyl Monolayer
by Synchrotron Soft X-ray. Langmuir,14,
20, 5673-5675, American Chemical Society
S. J. Oh, Y.-S. Choi, S. Hwangbo,
S. C. Bae, J. K. Ku, J. W. Park (1998) Structure
and phosphodiesterase activity of Bis-Tris
coordinated lanthanide(III) complexes. Chem.
Comm., 2189-2190. RSC
J. H. Moon, Y.-H. La, J. Y. Shim,
B. J. Hong, K. J. Kim, T.-H. Kang, B. Kim, H. Kang,
J. W. Park (2000) Selective cleavage
of the carbon-halide bond in substituted benzaldimine
monolayers by synchrotron soft X-ray. Langmuir,
16,6, 2981-2984. Am. Chem. Soc.
Hong Jin Kim, Joong Ho Moon, Joon
Won Park (2000) A Hyperbranched Poly(ethyleneimine) Grown
at the Surfaces. J. of Colloid and Interface
Science, Academic.
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