Sung Ho Ryu, Ph.D.

Professor
Department of Life Science
Division of Molecular and Life Sciences
Signal transduction & Proteomics

Publications Abstract
E-mail sungho@postech.ac.kr
Phone +82-54-279-2292(office)
          +82-54-279-5989(lab.)
Laboratory Signaling Proteome lab.

Profile |  Research Interests  |  Selected Publications

Profile

Research Interests

Signal Transduction
When extracellular signals, such as neurotransmitters, hormones, and growth factors, bind to their specific receptors, phospholipids breakdown is one of the immediate responses. The resulting breakdown products become second messengers to amplify the extracelluar signals, then the signals from the second messengers are transcended to various effector molecules, which, in turn, transduce their signals to next signaling mediators, and so forth. The final effects of the signal transduction would be one or more of the many cellular phenomena, such as proliferation, differentiation, or apoptosis.
The major research goals of the Signal Transduction Laboratory is the identification of the mechanism of signal transduction pathway mediated by phospholipases. Based on the substrate specificity and the produced second messengers, the phospholipases are classified to A, C, and D. Our lab has focused on the identification of the regulatory mechanisms of and the discovery of new molecular mediators for the signal transduction of phospholipase C and D. These studies include following studies. Purification of core signaling molecules such as phospholipases and its kinetic studies among various subtypes of the phospholipases; identification of binding proteins and in vitro molecular binding studies between phospholipases and their putative effectors; establishing transient and permanent cell lines expressing signal transduction-related molecules for in vivo studies. The accumulations of knowledges and experiences concerning the signal transduction pathway for the last decade allow us to expand our realm of research, not only in width but in depth, to the molecular libraries, such as synthetic peptides for the discovery of agonists or antagonists, which ultimately lead to the developments of new medicines. Recently, to provide valuable informations for the basic researchers as well as ideas for the industrial application in the field of signal transduction, we are going to characterize molecularly SMMC (signaling molecule multicomplex) which composes of signal machinery by using PMT (proteome molecular technology).

Selected Publications 

Seo, J.K., Choi, S.Y., Kim, Y., Baek, S.H., Kim, K.T., Chae, C.B., Suh, P.G., and Ryu, S.H. (1997) "A peptide with unique receptor specificity: stimulation of phosphoinositide hydrolysis and induction of superoxide generation in human neutrophils" J. Immunol. 158, 1895-1901.
1. Lee, T.G., Lee, Y.H., Kim, J.H., Kim, H.S., Suh, P.G., and Ryu, S.H. (1997) "Immunological identification of cholesterol ester hydrolysis in the steroidigenic tissues, adrenal glands and testis", Biochim. Biophys. Acta, 1346, 103-108
2. Lee, T.G., Park, J.B., Lee, S.D., Hong, S.B., Kim, J.H., Kim, Y., Yi, K.S., Bae, S., Hannun, Y., Obeid, L.M., Suh, P.G., Ryu, S.H (1997) "Phorbol myristate actetate-dependent association of protein kinase C-α with phospholipase D1 in intact cells" Biochim. Biophys. Acta, 1347, 199-204
3. Seo, J.K., Bae, Y.S., Song, H.B., Baek, S.H., Kim, B.S., Choi, W.S., Suh, P.G., and Ryu, S.H. (1998) "Distribution of the receptor for a novel peptide stimulating phosphoinositide hydrolysis in human leukocytes" Clin Biochem 31, 137-141.
4. Kim, J.H., Lee, S.D., Han, J.M., Lee, T.G., Kim, Y., Park, J.B., Lambeth, J.D., Suh, P.G. and Ryu, S.H. (1998) "Activation of phospholipase D1 by direct interaction with ADP-ribosylation factor 1 and RalA" FEBS Lett. 430, 231-235.
5. Kim, Y., Kim, J.E., Lee, S.D., Lee, T.G., Kim, J.H., Park, J.B., Han, J.M., Jang, S.K., Suh, P.G. and Ryu, S.H. (1999) "Phospholipase D1 is located and activated by protein kinase Cα in the plasma membrane in 3Y1 fibroblast cell" Biochim. Biophys. Acta 1436, 319-330.
6. Bae, Y.S., Ju, S.A., Kim, J.Y., Seo, J.K., Baek, S.H., Kwak, J.Y., Kim, B.S., Suh, P.G., and Ryu, S.H. (1999) "Trp-Lys-Tyr-Met-Val-dMet stimulates superoxide generation and killing of Staphylococcus aureus via phospholipase D activation in human monocytes" J. Leukoc. Biol. 65, 241-248.
7. Kim, J.H., Lee, S.D., Park, J.B., Lee, T.G., Kim, Y., Suh, P.G. and Ryu, S.H. (1999) "Phospholipase D1 in caveolae: Regulation by protein kinase Cα and caveolin-1" Biochemistry 38:3763-3769.
8. Kim, Y., Han, J.M., Park, J.B., Lee, S.D., Oh, Y.S., Chung, C., Lee, T.G., Kim, J.H., Park, S.K., Yoo, J.S., Suh, P.G. and Ryu, S.H. (1999) "Phosphorylation and activation of phospholipase D1 by protein kinase C in vivo: Determination of multiple phosphorylation sites" Biochemistry 38:10344-10351.
9. Kim, J.H., Kim, Y., Lee, S.D., Lopez, I., Arnold, R.S., Lambeth, J.D., Suh, P.G. and Ryu, S.H. (1999) "Selective activation of phospholipase D2 by unsaturated fatty acid" FEBS Lett. 454:42-46.
10. Kim, J.H., Lee, B.D., Lee, S.D., Kim, Y., Suh, P.G. and Ryu, S.H. (1999) "Cytosolic phospholipase A2-mediated regulation of phospholipase D2 in leukocyte cell lines" J. Immunol. 163, 5462-5470.
11. Bae, Y.S., Kim, Y. Kim, J.H., Suh, P.G. and Ryu, S.H. (1999) "Trp-Lys-Tyr-Met-Val-D-Met is a chemoattractant for human monocytes" J. Leukoc. Biol., 66, 915-922.
12. Bae, Y.S., Kim, Y.D., Kim, J.H., Lee, T.G., Kim, Y., Suh, P.G. and Ryu, S.H. (2000) "Independent funicioning of cytosolic phospholipase A2 and phospholipase D in the superoxide formation of human monocytes" J. Immunol., 164(8):4089-4096.
13. Lee, B.D., Kim, J.H., Lee, S.D., Kim, Y., Suh, P.G. and Ryu, S.H. (2000) "Hydrogen peroxide activates phospholipase D2 in L1210 cells" J. Leukoc. Biol., 67(5), 630-636.
14. Kim, Y. Han, J.M., Han, B.R., Lee, K.A., Kim, J.H., Lee, B.D., Jang, I.H., Suh, P.G. and Ryu, S.H. (2000) "Phospholipase D1 is phosphorylated and activated by protein kinase C in caveolin- and cholesterol-enriched microdomains within the plasma membrane" J. Biol. Chem. 275(18): 13621-13627.
15. Park, J.B., Kim, J.H., Kim, Y., Ha, S.H., Kim, J.H., Yoo, J.S., Du, G., Frohman, M.A., Suh, P.G., and Ryu, S.H. (2000) "Cardiac phospholipase D2 localizes to sarcolemmal membranes and is inhibited by alpha actinin in an ARF-reversible manner" J. Biol. Chem. 275, 21295-21301
16. Lee, S.D., Lee, B.D., Han, J.M., Kim, J.H., Kim, Y., Suh, P.G., and Ryu, S.H. (2000) "Phospholipase D2 activity supresses hydrogen peroxide-induced apoptosis in PC12 cells" J. Neurochem. 75(3), 1053-1059.
17. Lee, S.D., Lee, B.D., Kim, Y., Suh, P.G. and Ryu, S.H. (2000) "Bradykinin activates phospholipase D2 via protein kinase Cδ in PC12 cells" Neurosci. Lett. 294(2):130-2.
18. Jang, I.H., Kim, J.H., Lee, B.D., Bae, S.S., Park, S.K., Suh, P.G, and Ryu, S.H. (2001) "Localization of phospholipase C-γ1 signaling in caveolae: Importance in EGF-induced phosphoinositide hydrolysis but not in tyrosine phosphorylation" FEBS Lett. 491(1,2), 4-8. (Cover story)
19. Lee, S.M., Park, J.B., Kim, J.H., Kim, J.H., Kim, Y., Shin, K.J., Lee, J.S., Ha, S.H., Suh, P.G. and Ryu, S.H. (2001) "Actin directly interacts with phospholipase D inhibiting activity" J. Biol. Chem. 276(30), 28252-60.
20. Bae,  Y.S., Bae, H.J., Kim, Y.D., Lee, T.G., Suh, P.G. and Ryu, S.H. (2001) "Identification of novel chemoattractant peptides for human leukocytes" Blood, 97(9), 2854-62.
21. Lee, C.S., Bae, Y.S., Lee, S.D., Suh, P.G. and Ryu, S.H. (2001) "ATP-induced mitogenesis is modulated by phospholipase D2 through extracellular signal regulated protein kinase dephosphorylation in rat pheochromocytoma PC12 cells" Neurosci. Lett. 313(3), 117-20.
22. Bae, Y.S., Kim, Y., Park, J.C., Suh, P.G. and Ryu, S.H. (2002) "The synthetic chemoattractant peptide, Trp-Lys-Tyr-Met-Val-D-Met, enhances monocyte survival via PKC-dependent Akt activation" J. Leukoc. Biol. 71(2), 329-38.
23. Lee, S.M., Kim, J.H., Lee, C.S., Kim, J.H., Kim, Y., Heo, K., Ihara, Y., Goshima, Y., Suh, P.G. and Ryu, S.H. (2002) “Collapsin response mediator protein-2 inhibits neuronal phospholipase D2 activity by direct interaction" J. Biol. Chem. 277(8), 6542-49.
24. Kim, J.H., Lee, S.M., Kim, J.H., Lee, T.G., Hirata, M., Suh, P.G. and Ryu, S.H. (2002) "Phospholipase D2 directly interacts with aldolase via its PH domain" Biochemistry 41(10), 3414-21.
25. Han, J.M., Kim, J.H., Lee, B.D., Lee, S.D., Lee, S.M., Cho, W.H., Ohba, M., Kuroki, T., Suh, P.G. and Ryu, S.H. (2002) "Protein kinase Cδ-dependent phosphorylation and activation of phospholipase D2 in rat pheochromocytoma PC12 cells" J. Biol. Chem. 277(10), 8290-97.
26. Han, J.M., Y, Kim, Lee, J.S., Lee, C.S., Lee, B.D., Obah, M., Kuroki, T., Suh, P.G. and Ryu, S.H. (2002) “Localization of Phospholipase D1 to Caveolin-enriched Membrane via Palmitoylation : Implications for Epidermal Growth Factor Signaling”  Mol. Biol. Cell. 13(11), 3976-88.
27. Bae, Y.S., Lee, T.G., Park, J.C. Hur, J.H., Kim, Y.D., Heo, K., Suh, P.G. and Ryu, S.H. (2003) “Identification of a compound that directly stimulates phospholipase C activity“ Mol. Pharmacol., 63(5), 1043-50.
28. Kim, J.H., Lee, S., Park, J.B., Lee, S.D., Kim, J.H., Ha, S.H., Hasumi, K., Endo, A., Suh, P.G. and Ryu, S.H. (2003) "Hydrogen peroxide induces association between glyceraldehyde 3-phosphate dehydrogenase and phospholipase D2 to facilitates phospholipase D2 activation in PC12 cells" J. Neurochem. 85(5), 1228-36
29. Jang, I.H., Lee, S.M., Park, J.B., Kim, J.H., Lee, C.S., Hur, E.M., Kim, K.T., Suh, P.G. and Ryu, S.H. (2003) "Direct interaction of phospholipase Cγ1 with phospholipase D2 is important for EGF signaling" J. Biol. Chem. 278(20), 18184-90.
30. Bae, Y.S., Park, J.C. Suh, P.G. and Ryu, S.H. (2003) "Differential Signaling of Formyl Peptide Receptor Like 1 by Trp-Lys-Tyr-Met-Val-Met-CONH₂ and Lipoxin A4 in Human Neutrophils" Mol. Pharmacol., 64(3), 721-30.
31. Bae, Y.S., Song, J.Y., Kim, Y.D., He, R., Ye, R.D., Suh, P.G. and Ryu, S.H. (2003) "Differential activation of formyl peptide receptor signaling by peptide ligands"  Mol. Pharmacol., 64(4), 841-7.
32. Bae, Y.S., Park, E.Y., Kim, Y.D., He, R., Ye, R.D., Kwak, J.Y., Suh, P.G. and Ryu, S.H. (2003) "Novel chemoattractant peptides that differentially stimulate human leukocytes" Biochem. Pharmacol., 66(9), 1841-51.
33. Park, J.B., Lee, H.Y., Lee, C.S., Kim, I.S., Lee, B.D., Jang, I.H., Jung, Y.W., Oh, Y.S., Han, M.Y., Jensen, O.N., Roepstorff, P., Suh, P.G. and Ryu, S.H. (2004) "Regulation of phospholipase D2 through GTP-dependent interaction with dynamin" Adv. Enzyme Regul. 44(1), 249-64.
34. Lee, H.Y., Park, J.B., Jang, I.H., Chae, Y.C., Kim, J.H. Kim, I.S., Suh, P.G. and Ryu, S.H. (2004) "Munc-18-1 directly interacts with the phox homology domain of phospholipase D to inhibit its activity in an epidermal growth factor-reversible manner" J. Biol. Chem. 279(16), 16339-48.
35. Chae, Y.C., Lee, H.Y., Heo, K., Kim, J.H., Kim, J.H., Suh, P.G. and Ryu, S.H. (2005) "Inhibition of Muscarinic Receptor-linked Phospholipase D Activation by Association with Tubulin" J. Biol. Chem. 280(5), 3723-30.
36. Kim, J.H., Kim, J.H., Ohba, M., Suh, P.G. and Ryu, S.H. (2005) "Novel Functions of the Phospholipase D2 - Phox Homology Domain in Protein Kinase Cz Activation" Mol. Cell. Biol. 25(8), 3194-208.
37. Lee, J.S., Kim, J.H., Jang, I.H., Kim, H.S., Han, J.M., Kazlauskas, A., Yagisawa, H., Suh, P.G. and Ryu, S.H. (2005) "Phosphatidylinositol 3,4,5-trisphosphate Specifically Interacts with the Phox Homology Domain of Phospholipase D1 and Stimulates its Activity" J. Cell. Sci., 118(19), 4405-13.
38. Lee, B.D., Kim, S.M., Hur, E.M., Park, Y.S., Kim, Y.H., Lee, T.G., Kim, K.T., Suh, P.G. and Ryu, S.H. (2005) "Leumorphin has anti-apoptotic effect by activating protein kinase B and extracellular signal-related protein kinase in Rat Pheochromocytoma PC12 cells" J. Neurochem., 95(1), 56-67.
39. Baek, M.C., Kim, S.J., Yea, K.M., Kim, Y.D., Lee, B.D., Kim, J.Y., Lee, H.J. Kang, M.H., Choi, S.K., Kim, J.I., Lee, T.G., Suh, P.G. and Ryu, S.H. (2006) "Ligand profiling and identification (LPI) technology for searching bioactive ligands" Proteomics 6(6), 1741-9.
40. Kim, Y.D., Lee, B.D., Kim, Y.K., Bae, Y.S., Lee, T.G., Suh, P.G. and Ryu, S.H. (2006)  "Pituitary adenylate cyclase-activating peptide 27 is a functional ligand for formyl peptide receptor like-1" J. Immunol. 176(5), 2969-75.
41. Lee, C.S., Kim, I.S., Park, J.B., Lee, M.N., Lee, H.Y., Suh, P.G. and Ryu, S.H. (2006) "The phox homology domain of Phospholipase D activates dynamin GTPase activity and accelerates EGFR endocytosis" Nature Cell Biol. 8(5), 477-484.
42. Kim, J.H., Kim, H.W., Jeon, H., Suh, P.G. and Ryu, S.H. (2006) "Phospholipase D1 regulates cell migration in a lipase activity-independent manner" J. Biol. Chem. 281(23), 15747-56.
43. Ha, S.H., Kim, D.H., Kim, I.S., Kim, J.H., Lee, M.N., Lee, H.J., Kim, J.H., Jang, S.K., Suh, P.G. and Ryu, S.H. (2006) "PLD2 forms a functional complex with mTOR/raptor to transduce mitogenic signals" Cell. Signal. 18(12), 2283-91.
44. Lee, M.S., Yoo, S.A., Miyazawa, K., Cho, C.S., Suh, P.G., Kim, W.U. and Ryu, S.H. (2006) "Serum amyloid A binding to formyl peptide receptor-like 1 induces synovial hyperplasia and angiogenesis" J. Immunol., 177(8), 5585-94.

Division of Molecular & Life Sciences| POSTECH