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Objective & Scope
1. Characterization of biological phosphorus removal mechanism 2. Analysis of the microbial communities 3. Identification of PAOs Main Contents Phosphorus and nitrogen in water resources stimulate growth of algae and other photosynthetic aquatic life, which lead to accelerated eutrophication, excessive loss of oxygen resources, and undesirable changes in aquatic populations. Because there are several reasons or benefits, for utilizing biological nutrient removal (BNR) processes for the treatment of wastewaters, the biological system has been considered to be the most effective and ultimate method, and most widely adapted in real operations of nutrient removal. Among many nutrients in water, the removal of phosphorus compounds has been revealed as the more important solution of eutrophication prevention because nitrogen can be fixed by various methods such as biological or natural (lightning) fixation. Therefore, in this study, biological nutrient removal, especially biological phosphorus removal, in anaerobic/aerobic sequencing batch reactors fed with different carbon sources was studied. Metabolism of enhanced biological phosphorus removal (EBRP) in an anaerobic/aerobic sequencing batch reactor (SBR) was investigated when glucose was supplied as the sole carbon source. A probable metabolic model of PHA synthesis and phosphorus removal in a SBR supplied with glucose as the sole carbon source was proposed. Experiments works were carried out to investigate the relationships between PHA synthesis from acetage, stored glycogen and added glucose and the fate of added glucose in a lab-scale SBR fed with glucose and acetate mixture. pH effect on the competition between glycogen accumulating organism (GAO) and PAO was investigated through a characterization of activated sludge using proposed biochemical models. Several methods such as electron microscope analysis, quinone analysis, slot hybridization and 16S rDNA PCR cloning approach were used to analyze the microbial communities and to identify the PAO responsible for EBPR in the anaerobic/aerobic SBRs with glucose, acetate and glucose/acetate mixture. References 1) Biological Nutrient Removal in Anaerobic/Aerobic Sequencing Batch Reactors Supplied with Different Carbon Sources Che Ok Jeon, Thesis of Doctor.(2000), POSTECH, KOREA 2) Characteristics of enhanced biological phosphorus removal of activated sludge in anaerobic/aerobic sequencing batch reactors C.O. Jeon, D.S. Lee, and J.M. Park, J. Kor. Soc. Env. Eng., 22(8), 1535 (2000) 3) Microbial communities of activated sludge in an anaerobic/aerobic sequencing batch reactor using slot hybridization C.O. Jeon, K.-J. Shin, D.S. Lee, P.-G. Suh, and J.M. Park, J. Kor. Soc. Env. Eng., 22(5), 939 (2000) 4) Biological nutrient removal by enhancing anoxic phosphate uptake D.S Lee, C.O. Jeon, and J.M. Park, J. Kor. Soc. Env. Eng., 22(5), 861 (2000) 5) Enhanced biological phosphorus removal in a sequencing batch reactor supplied with glucose as a sole carbon source C.O. Jeon and J.M. Park, Water Res., 34(7), 2160 (2000) 6) Morphological characteristics of microbial sludge performing enhanced biological phosphorus removal in a sequencing batch reactor fed with glucose as sole carbon source C.O. Jeon, D.S. Lee, and J.M. Park, Water Sci. Technol., 41(12), 79 (2000) 7) Enhanced biological phosphorus removal in an anaerobic/aerobic sequencing batch reactor: Effect of pH C.O. Jeon, D.S. Lee, and J.M. Park, Water Env. Res., 73(3), 301 (2001) 8) Enhanced biological phosphorus removal in an anaerobic/aerobic sequencing batch reactor: Characteristics of carbon metabolism C.O. Jeon, D.S. Lee, and J.M. Park, Water Env. Res., 73(3), 295 (2001) 9) Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system D.S. Lee, C.O. Jeon, and J.M. Park, Water Research, 35(16), 206 (2001) 10) Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor C.O. Jeon, D.S. Lee, and J.M. Park, Water Research, 37(9), 2195 (2003) 11) Microbial communities of activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor supplied with glucose C.O. Jeon, S.H. Woo, and J.M. Park, J. Microb. Biotechnol., in press. |
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