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Objective & Scope
1. Screening of PHA degrading bacteria 2. Characterization of biological mechanism of PHA degradation 3. Modeling of biological PHA degradation 4. Development of efficient bioreactor process Main Contents Bioremediation, the use of microbial processes to degrade environmental contaminants, is one of the emerging technology for the remediation of sites contaminated with hazardous compounds. Among the various bioremediation technologies, a slurry bioreactor process is considered to be one of the most fasted treatment. However, the use of conventional slurry bioreactor has been limited to silt or clay because large particles such as sand cannot be easily suspended in this reactor. Hence, sand has been usually separated from silty and clayey soil and treated by washing method. However, the washing method requires extra equipment and large amount of water. Therefore, in this study, the drum bioreactor system was developed to simultaneously treat the whole contaminated soil without removal of sand from soil and separate washing. This study was concentrated on the development of strategy to treat soil with concept of soil two-phase in a drum bioreactor. The optimization of water addition, the treatability of soil, and the simulation of soil two-phase system were performed. The PAH-degrading microorganisms were isolated from soil. These mixed microorganisms could degrade naphthalene, fluorene, phenanthrene, anthracent, and pyrene. A kinetic model was developed to simulate the biodegradation of phenanthrene in a soil two phase system. In the drum bioreactor system, the oxygen transfer seemed to be not the rate-limiting step. However, the mass transfer rate of phenanthrene from soil to water was comparable to the bioreaction rate. This could be also demonstrated by the simulation results. References 1) Development of bioreactor system for the treatment of soil contaminated with polycyclic aromatic hydrocarbons Seung Han Woo, Thesis of Doctor(1998), POSTECH, KOREA 2) Development of bioreactor system for the treatment of PAH-contaminated soil S.H. Woo, and J.M. Park, Biochem. Eng., 10(1), 24 (1996) 3) Bioremediation of PAH-contaminated soil using a mobile bioreactor system S.H. Woo, and J.M. Park, Biotechnol. News, (1996) 4) Estimation of oxygen transfer in soil slurry bioreactor S.H. Woo and J.M. Park, Biotech. Tech., 11, 713 (1997) 5) Evaluation of drum bioreactor performance used for decontamination of soil polluted with polycyclic aromatic hydrocarbons S.H. Woo and J.M. Park, J. Chem. Tech. Biotech. 74, 937 (1999) 6) Evaluation of the interaction between biodegradation and sorption of phenanthrene in soil-slurry systems S.H. Woo, J.M. Park, and B.E. Rittmann, Biotechnol. Bioeng., 73(1), 12 (2001) Related Patents 1) Methods and equipments for the biological treatment of contaminated soils, K.P. Lee, C.S. Song, S.H. Woo, and J.M. Park, Korea Patent (1999) |
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