POSTECH RISD

(Rice T-DNA Insertion Seqence Database)

We have generated T-DNA insertional and activation tagging lines in japonica rice Oryza sativa cv. Dongjin or Hwayoung. The vectors used are pGA2707 (GUS trapping vector), pGA2717 (GUS and GFP trapping vector), and activation tagging vectors pGA2715 and pGA2772 (Jeon et al., 2000, Jeong et al., 2002). Flanking sequences of each T-DNA were determined by inverse-PCR or TAIL PCR (An et al., 2003, Jeong et al., 2006). The database can be searched at http://signal.salk.edu/cgi-bin/RiceGE or
http://rapdb.dna.affrc.go.jp/cgi-bin/gbrowse/IRGSP40/?name=chr1:1..20000.

To request seeds, select the line numbers and send the list to sookan@postech.ac.kr. We will inform you whether we have enough seeds to send them out. We provide 15-30 grains from the primary transgenic plants. If the seed number is less than 50 in our stock, we need to amplify the seeds before sent them out. If you are willing to wait for the seed amplification, please request us to amplify the seeds. It will take several months. For some lines, we have T2 seeds of individual plants (usually 5 to 10) and some of them are genotyped.

Fill out the MTA form (download) in duplicate and send it to Gynheung An, Department of Life Science, Pohang University of Science and Technology, Pohang, 790-784, Republic of Korea. If you are ordering from USA or Australia, you will need to obtain an import permit from your government before we send you seeds. Other countries may also restrict the seed import.

We charge for a handling fee of US $100 and $50 per line. When we have T2 seeds and if you request their seeds, we will charge extra $50 per line. We may restrict the number of lines to be less than 100 per principal investigator in a given year.